Coding

Part:BBa_K3096007:Design

Designed by: Famke Baeuerle, Lina Widerspick, Patrick Mueller, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)


Type I CRISPR/Cas system surveillance complex Cascade ABCDE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For a functioning CRISPR system you need the nuclease Cas3 and a crRNA


Source

E.coli K12/ MG1655

References